A Simple Radial Immunodiffusion Method for Assay of f32-Microglobulin in Serum
نویسندگان
چکیده
In this method for clinical measurement of /32-microglobulin, a 10 g/L agarose gel containing anti-/32-microglobulin is used, with subsequent staining with Coomassie Blue. Although the method is slow (requiring 30 h), it is inexpensive, reliable, and accurate over the range of ito 10 mg/L, and is useful in the determination of f32-microglobulin in serum and cerebrospinal fluid. A modified procedure in which staining with silver is used may be sufficiently sensitive for the clinical assay of urine.
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